Coding

Part:BBa_K2707013:Design

Designed by: Weifeng Lin   Group: iGEM18_NPU-China   (2018-10-05)


BFD F464W


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 759
    Illegal NgoMIV site found at 1221
    Illegal AgeI site found at 223
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

we use point mutate to improve the function of the protein.


Source

The organism is Pseudomonas putida (Arthrobacter siderocapsulatus)

References